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Overview
The uncertainty associated with the supply of petroleum has increased interest and research in feasible alternative fuels. Although biofuels have emerged as an attractive alternative to fossil fuels, large scale development is currently unfeasible. An important aspect of alternative fuels is the development of renewable fuel characterization, processes, and contaminant analysis using robust analytical methods. Ion chromatography (IC) is a proven technique for providing fast and reliable answers to biofuel workflows from research to production.
This webinar will focus on the need for a fast and robust approach for high resolution of carbohydrates in feedstock. There are several liquid chromatography (LC) methods in the industry for analysis of sugars; however, development of a robust analytical method for accurate quantification of sugars in biofuel feedstock is challenging. This webinar will focus on understanding high-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) technology for the analysis of carbohydrates in feedstock. This involves optimizing method parameters, including column chemistry, to get fast, efficient separation of mono- and disaccharides with good resolution, linearity, and accuracy over a broad dynamic range.
We will also focus on the analysis of saccharides and cellobiose during the enzymatic or chemical hydrolysis of complex carbohydrates in biomass materials. Depending on the hydrolysis condition, some residual sucrose and cellobiose may be present. While cellulose in the biomass is hydrolyzed to cellobiose by cellulase, beta-glucosidase is required for further hydrolysis. If beta-glucosidase activity is insufficient, cellobiose and other soluble sugars will accumulate and compromise fermentation efficiency. We will provide examples on the importance of monitoring cellobiose, other saccharides, and their impact on the fermentation process.
Learning Objectives
HPAE-PAD methods for carbohydrate analysis in feedstock
How parameters are important in validating methods to ensure accurate quantification
Who should attend?
Scientists interested in biofuels
Lab managers who are considering performing this analysis
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